2014年9月22日 讯 /生物谷BIOON/ --细胞疗法，即利用完好无损的细胞来治疗疾病，其是当前医学领域治疗疾病的一种重要方法，但是目前仍然存在很多障碍，比如不能使功能细胞达到机体的病灶或者使机体产生抗性等；近日，发表在国际杂志Magnetic Resonance in Medicine上的一篇研究论文中，来自加利福尼亚大学的研究人员利用全氟碳化合物(PFC)示踪技术和非侵入性磁共振成像成技术成功地追踪了结直肠癌患者机体中被注射的治疗性免疫细胞。

博士Eric T. Ahrens表示，起初我们想观察这种技术联合作用对于新型细胞疗法的效果，而我们可以通过反馈细胞活性、改善剂量等途径来改善细胞疗法的效率；当前并没有有效的方法对人类机体中的细胞进行成像，早先可以利用基于金属离子的血管MRI对比制剂和放射性同位素来成像，但是其在体内就不能够对细胞进行有效区分了。

研究者表示，这项研究中他们利用全氟碳化合物(PFC)示踪技术和非侵入性磁共振成像成技术进行结合来直接检测标记细胞的氟原子，自然状态下氟原子在机体中的浓度极低，这就可以利用MRI技术对氟标记的细胞进行观察；而本文中研究者首次通过患者的白细胞制备了被修饰和标记的树突细胞，随后研究者将这些细胞注入到4期结直肠癌患者的机体中来刺激机体抗癌T细胞免疫反应。

Ahrens表示，显影剂技术已经广泛用于对任何类型细胞的标记中，其是一种成像平台可以进行多种应用，对于加速相关疾病的疗法非常关键；细胞的非侵入性追踪技术可以帮助降低障碍难度，比如新型的干细胞疗法就可以部分获得批准来用于开发改善移植细胞生存期的技术，最后研究者表示，未来细胞疗法或许可以作为一种最佳疗法来有效治疗患者的疾病及改善患者的健康状况。

doi:10.1002/mrm.25454

Clinical cell therapy imaging using a perfluorocarbon tracer and fluorine-19 MRI

Eric T. Ahrens1,*, Brooke M. Helfer2, Charles F. O'Hanlon2 andClaudiu Schirda3

Purpose Cellular therapeutics are emerging as a treatment option for a host of serious human diseases. To accelerate clinical translation, noninvasive imaging of cell grafts in clinical trials can potentially be used to assess the initial delivery and behavior of cells. Methods The use of a perfluorocarbon (PFC) tracer agent for clinical fluorine-19 (19F) MRI cell detection is described. This technology was used to detect immunotherapeutic dendritic cells (DCs) delivered to colorectal adenocarcinoma patients. Autologous DC vaccines were labeled with a PFC MRI agent ex vivo. Patients received DCs intradermally, and 19F spin-density-weighted MRI at 3 Tesla (T) was used to observe cells. Results Spin-density-weighted 19F images at the injection site displayed DCs as background-free “hot-spot” images. 19F images were acquired in clinically relevant scan times (<10 min). Apparent DC numbers could be quantified in two patients from the 19F hot-spots and were observed to decrease by ∼50% at injection site by 24 h. From 3T phantom studies, the sensitivity limit for DC detection is estimated to be on the order of ∼105 cells/voxel in this study. Conclusion These results help to establish a clinically applicable means to track a broad range of cell types used in cell therapy. Magn Reson Med, 2014. © 2014 The Authors. Magnetic Resonance in Medicine Published by Wiley Periodicals, Inc. on behalf of International Society of Medicine in Resonance. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited.

转载自生物谷

http://news.bioon.com/article/6659137.html